Science

BACLIB measures unique lipid molecules from microbial membranes. In Gram-negative bacteria, lipid A molecules are the primary analytes. Analogous lipids are analyzed in Gram-positive bacteria and fungi.

BACLIB measures lipids using a MALDI instrument, the de-facto standard for clinical microbiology. Samples are prepared for analysis right on a MALDI plate.

In one hour, BACLIB reports bacterial and fungal species IDs and specific antimicrobial resistances.

Seconds of touch time

BACLIB’s simple prep requires only seconds of handling per sample, with clinic-friendly reagents:

1. Apply samples to MALDI plate.
2. Heat plate for 30 min.
3. Rinse with water.
4. Apply MALDI matrix.
Samples are ready for MALDI.

Lipid fingerprints

BACLIB measures unique lipid molecules from microbial membranes.

In Gram-negative bacteria, lipid A molecules are the primary analytes. Analogous lipids are analyzed in Gram-positive bacteria and fungi.

Unique lipid A structures in Gram-negative bacteria

Escherichia coli


Pseudomonas Aeruginosa


Salmonella typhimurium


Broad coverage

The number of species in BACLIB’s library is growing all the time.
Highlights include:

  • The ESKAPE pathogens: E. faecium, S. aureus, K. pneumoniae, A. baumannii, P. aeruginosa, Enterobacter.
  • Common causes of sepsis: S. aureus, S. epidermidis, E. coli, S. pneumoniae, C. albicans, and others.
  • Common UTI pathogens: Proteus mirabilis, Staphylococcus, and others.
  • CDC Emerging Infections: C. difficile, Shigella, Salmonella.
  • Biodefense: Y. pestis, F. tularensis, Burkholderia, Brucella.
  • Twenty-three Candida species are uniquely identified, including C. albicans and C. auris.

Accurate results

Sensitivity and specificity of BACLIB species IDs are typically  > 90%. In many cases, BACLIB reliably distinguishes species that confound other tests. The Below ROC graphs show BACLIB’s typically outstanding true positive vs false positive rates, for two difficult diagnostic challenges.

Detecting colistin resistance. Shown is BACLIB’s typical performance in detecting the colistin resistance-conferring mcr-1 plasmid in E. coli.

Distinguishing Candida species. Each graph shows BACLIB’s ability to distinguish one Candida species from others in the genus.