From Our Blog

Pataigin Appoints Deborah Elvins as Strategic Advisor

SEATTLE, WA – 30 March, 2022 – Microbiology diagnostics company Pataigin today appointed Deborah A. Elvins as an advisor to the company on business strategy and other business matters.

Elvins is a retired life sciences lawyer with an extensive life sciences background. That background includes senior roles in two publicly traded biotech companies: VP Legal Affairs at Dendreon and Acting General Counsel for Cascadian Therapeutics. She was also a partner in Adkins Black LLP, a boutique law firm focused on life sciences, where her practice included working directly with senior management on a wide range of legal, regulatory, and business issues. Prior to Adkins Black, Elvins was a partner at Stoel Rives LLP, a large Pacific Northwest law firm where, in addition to her law practice, she held senior management roles with the firm.

Continue readingPataigin Appoints Deborah Elvins as Strategic Advisor

A Novel Lipid-Based MALDI-TOF Assay for the Rapid Detection of Colistin-Resistant Enterobacter Species

Smith RD, McElheny CL, Izac JR, Gardner FM, Chandler CE, Goodlett DR, Doi Y, Johnson JK, Ernst RK. Microb Sectrum 2022 Feb 2.

Enterobacter species are classified as high-priority pathogens due to high prevalence of multidrug resistance from persistent antibiotic use. For Enterobacter infections caused by multidrug-resistant isolates, colistin (polymyxin E), a last-resort antibiotic, is a potential treatment option. Treatment with colistin has been shown to lead to emergence of polymyxin resistance. The primary mechanism for colistin resistance is modification of terminal phosphate moieties of lipid A, leading to decreased membrane electronegativity and reducing colistin binding affinity. Detection of these modifications, including the addition of phosphoethanolamine and 4-amino-4-deoxy-l-arabinose (Ara4N), can be used for prediction of colistin resistance using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The objective of this study was to identify lipid A markers for colistin resistance in Enterobacter species and Klebsiella aerogenes (formerly Enterobacter aerogenes). … Continue readingA Novel Lipid-Based MALDI-TOF Assay for the Rapid Detection of Colistin-Resistant Enterobacter Species

Direct-From-Specimen Results to be Presented at ECCMID 2022 Conference

LISBON, Portugal: Results will be presented from a study of Pataigin’s rapid bacterial ID assay, direct from clinical specimens.

At the 32nd European Congress of Clinical Microbiology & Infectious Diseases (ECCMID), Richard D. Smith will present, “Fast lipid analysis technique (FLAT) a rapid, direct from urine diagnostic assay for identification of Gram-negative pathogens.”

Continue readingDirect-From-Specimen Results to be Presented at ECCMID 2022 Conference

Rapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay

Smith RD, Izac JR, Ha M, et al. Access Microbio. 2021 Dec 17

Mobilized colistin resistance (mcr) genes confer resistance to colistin, a last-resort antibiotic for multidrug-resistant Gram-negative infections. In this case report, we describe a novel lipid-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) diagnostic used to rapidly identify an mcr-1-positive Escherichia coli directly from a patient with a urinary tract infection without the need for ex vivo growth. … Continue readingRapid identification of mcr-1-positive Escherichia coli from patient urine using a novel lipid-based MALDI-TOF-MS assay

Pataigin Introduces First Multiplex Lipid Microbiology Kit

VIENNA, Austria, BALTIMORE Maryland, and Seattle, Washington – 13 July 2021: At the 31st European Congress of Clinical Microbiology & Infectious Diseases (ECCMID, www.eccmid.org), Pataigin introduced the BACLIB Rapid Lipid Extraction Kit for microbial identification and colistin resistance detection.

The patented BACLIB Kit is unique in determining bacterial species ID and colistin resistance with a single assay. Superior to competing lipid microbiology kits, BACLIB requires less than a minute of hands-on time per sample, with no centrifugation or complex handling steps.

In contrast to competing kits that only identify a single species, the multiplex BACLIB Kit identifies and determines colistin resistance for all the ESKAPE pathogens. The BACLIB Kit includes access to custom software for ID/resistance determination. Time-to-result is only 30 minutes.

Continue readingPataigin Introduces First Multiplex Lipid Microbiology Kit

Pataigin Awarded NIH Grant to Improve Antimicrobial Stewardship

Today, the US NIH awarded Pataigin a research grant to further development of Pataigin’s Rigel antimicrobial susceptibility test.

“We thank the NIH for supporting this research,” said Pataigin CEO Erik Nilsson. “Rigel promises to reduce medical costs while saving lives now lost to infectious disease.”

The grant, entitled Isotopic Labeling Rapid Antimicrobial Susceptibility Testing, furthers development of a rapid and sensitive Rigel AST test using lipid microbiology technology. Rigel can determine appropriate antibiotic therapy in six hours, compared to days for conventional tests.

“Rigel is based on my lab’s longstanding research into lipid microbiology,” said Prof. Robert Ernst, the Dr. Paul and Mrs. Jean Corcoran Professor and Chair, Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore. “In this research, we extend lipid microbiology beyond microbial ID to antimicrobial susceptibility testing.” Prof. Ernst is a collaborator on the grant.

Bacteremia and sepsis remain major health problems with high mortality. Progression is often rapid, so that a single hour saved in diagnosis time can significantly reduce septicemia death rates. Faster tests are therefore needed to save lives. By significantly accelerating test results, Rigel assay offers the potential of improved clinical outcomes at lower cost to hospitals.

Continue readingPataigin Awarded NIH Grant to Improve Antimicrobial Stewardship

Pataigin Founder Ernst Presents Rapid Colistin Assay at World Microbe Forum

Today, Pataigin founder Prof. Robert Ernst presented results of a new assay that determines microbial ID and colistin resistance in one hour, direct from clinical specimens. The assay uses lipid microbiology with the FLAT extraction technique to allow colistin resistance to be rapidly determined without culture.

Polymyxins – polymyxin B and polymyxin E (colistin) are increasingly used worldwide to treat patients with multidrug-resistant (MDR) Gram-negative bacterial infections. However, there currently is no reliable test for colistin resistance, complicating colistin treatment and increasing risks to patients. Prof. Ernst demonstrated that Pataigin’s FLAT technique can be reliably used to identify colistin resistance. The rapid and accurate test presented by Prof. Ernst promises to improve the safety and efficacy of colistin therapy.

The talk was entitled “Rapid Lipid-Based MALDI-TOF Assay for the Rapid Detection of Colistin Resistance,” and was presented in World Microbe Forum session AAR104. Prof. Ernst is the Dr. Paul and Mrs. Jean Corcoran Professor and Chair, Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore.

Pataigin is developing lipid microbiology tests for bacterial ID, antimicrobial resistance, and antimicrobial susceptibility.

Continue readingPataigin Founder Ernst Presents Rapid Colistin Assay at World Microbe Forum

Deep-sea microbes as tools to refine the rules of innate immune pattern recognition

Gauthier AE, Chandler CE, Poli V, Gardner FM, et al. Sci Immunol. 2021 Mar 12

The assumption of near-universal bacterial detection by pattern recognition receptors is a foundation of immunology. The limits of this pattern recognition concept, however, remain undefined. As a test of this hypothesis, we determined whether mammalian cells can recognize bacteria that they have never had the natural opportunity to encounter. These bacteria were cultivated from the deep Pacific Ocean, where the genus Moritella was identified as a common constituent of the culturable microbiota. Most deep-sea bacteria contained cell wall lipopolysaccharide (LPS) structures that were expected to be immunostimulatory, and some deep-sea bacteria activated inflammatory responses from mammalian LPS receptors. However, LPS receptors were unable to detect 80% of deep-sea bacteria examined, with LPS acyl chain length being identified as a potential determinant of immunosilence. The inability of immune receptors to detect most bacteria from a different ecosystem suggests that pattern recognition strategies may be defined locally, not globally. … Continue readingDeep-sea microbes as tools to refine the rules of innate immune pattern recognition

Colistin heteroresistance is largely undetected among carbapenem-resistant Enterobacterales in the United States

Band V, Satola S, Smith R, et al. mBio 2021 Jan 26

Heteroresistance is a form of antibiotic resistance where a bacterial strain is comprised of a minor resistant subpopulation and a majority susceptible subpopulation. We showed previously that colistin heteroresistance can mediate the failure of colistin therapy in an in vivo infection model, even for isolates designated susceptible by clinical diagnostics. We sought to characterize the extent of colistin heteroresistance among the highly drug-resistant carbapenem-resistant Enterobacterales (CRE). We screened 408 isolates for colistin heteroresistance. These isolates were collected between 2012 and 2015 in eight U.S. states as part of active surveillance for CRE. Colistin heteroresistance was detected in 10.1% (41/408) of isolates, and it was more common than conventional homogenous resistance (7.1%, 29/408). Most (93.2%, 38/41) of these heteroresistant isolates were classified as colistin susceptible by standard clinical diagnostic testing. The frequency of colistin heteroresistance was greatest in 2015, the last year of the study. This was especially true among Enterobacter isolates, of which specific species had the highest rates of heteroresistance. Among Klebsiella pneumoniae isolates, which were the majority of isolates tested, there was a closely related cluster of colistin-heteroresistant ST-258 isolates found mostly in Georgia. However, cladistic analysis revealed that, overall, there was significant diversity in the genetic backgrounds of heteroresistant K. pneumoniae isolates. These findings suggest that due to being largely undetected in the clinic, colistin heteroresistance among CRE is underappreciated in the United States. … Continue readingColistin heteroresistance is largely undetected among carbapenem-resistant Enterobacterales in the United States

Published results: BACLIB Method IDs Pathogens and Colistin Resistance in One Hour

Scientific Reports today published a paper describing the FLAT rapid extraction method that allows identification of bacteria and fungi in one hour. Determination of colistin resistance in Gram-negative bacteria is also demonstrated. The paper was authored by Pataigin personnel in collaboration with the labs of BACLIB inventors Prof. Robert Ernst and Prof. David Goodlett. … Continue readingPublished results: BACLIB Method IDs Pathogens and Colistin Resistance in One Hour