Deep-sea microbes as tools to refine the rules of innate immune pattern recognition

Anna E. Gauthier, Courtney E. Chandler, Valentina Poli, Francesca M. Gardner, Aranteiti Tekiau, Richard Smith, Kevin S. Bonham, Erik E. Cordes, Timothy M. Shank, Ivan Zanoni, David R. Goodlett, Steven J. Biller, Robert K. Ernst, Randi D. Rotjan, Jonathan C. Kagan. Science Immunology. 2021 Mar 12; 6(57). doi.org/10.1126/sciimmunol.abe0531

The assumption of near-universal bacterial detection by pattern recognition receptors is a foundation of immunology. The limits of this pattern recognition concept, however, remain undefined. As a test of this hypothesis, we determined whether mammalian cells can recognize bacteria that they have never had the natural opportunity to encounter. These bacteria were cultivated from the deep Pacific Ocean, where the genus Moritella was identified as a common constituent of the culturable microbiota. Most deep-sea bacteria contained cell wall lipopolysaccharide (LPS) structures that were expected to be immunostimulatory, and some deep-sea bacteria activated inflammatory responses from mammalian LPS receptors. However, LPS receptors were unable to detect 80% of deep-sea bacteria examined, with LPS acyl chain length being identified as a potential determinant of immunosilence. The inability of immune receptors to detect most bacteria from a different ecosystem suggests that pattern recognition strategies may be defined locally, not globally.