Scientific Reports today published a paper describing the FLAT rapid extraction method that allows identification of bacteria and fungi in one hour. Determination of colistin resistance in Gram-negative bacteria is also demonstrated. The paper was authored by Pataigin personnel in collaboration with the labs of BACLIB inventors Prof. Robert Ernst and Prof. David Goodlett.
Rapid microbial identification and colistin resistance detection via MALDI-TOF MS using a novel on-target extraction of membrane lipids
Matthew Sorensen, Courtney E. Chandler, Francesca M. Gardner, Salma Ramadan, Prasanna D. Khot, Lisa M. Leung, Christine E. Farrance, David R. Goodlett, Robert K. Ernst & Erik Nilsson
Rapid infection diagnosis is critical to improving patient treatment and outcome. Recent studies have shown microbial lipids to be sensitive and selective biomarkers for identifying bacterial and fungal species and antimicrobial resistance. Practical procedures for microbial lipid biomarker analysis will therefore improve patient outcomes and antimicrobial stewardship. However, current lipid extraction methods require significant hands-on time and are thus not suited for direct adoption as a clinical assay for microbial identification. Here, we have developed a method for lipid extraction directly on the surface of stainless-steel matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plates, termed fast lipid analysis technique or FLAT, which facilitates the identification of bacterial and fungal species using a sub-60-minute workflow. Additionally, our method detects lipid A modifications in Gram-negative bacteria that are associated with antimicrobial resistance, including to colistin.